METHOD FOR CONTROLLING DNA TRANSCRIPTION REACTION

摘要

PROBLEM TO BE SOLVED: To provide a method for controlling DNA transcription reaction that can artificially start or stop DNA transcription reaction and can increase the transcription activity by adjusting the composition of the transcription reaction solution in no need of addition or removal of transcriptional factor protein.

SOLUTION: The addition of polyvalent ion/a hydrophilic polymer, anion or the like of specific concentration to the transcription reaction solution causes a large scale of high-order structural change in template DNA and the transcription activity is increased or decreased. In addition, the transcription activity can be ON/OFF-controlled by the discontinuous transfer (folding transfer). This invention is a method for starting or stopping the transcription reaction of the DNA by adding a substance inducing the folding transfer of the DNA to the transcription reaction solution including a long-chain DNA so that the concentration of the substance may reach in a prescribed range and the concentration of the substance inducing the folding transfer in the transcription reaction solution is selected in the range so that the unraveled long chain DNA may be converted to the unraveled coil structure or agglutinated structure.

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