A method for the high level expression of active lymphotoxin-beta receptor immunoglobulin chimeric proteins and their purification

摘要

Methods for the expression of improved yields of the active protein-immunoglobulin fusions having high affinity binding to its ligand, by culturing hosts transformed with DNA encoding the desired fusions in a culture system at a low temperature thereby minimizing the amount of misfolded or misbridged protein forms are disclosed. The host cells may be transformed mammalian, insect, yeast or bacterial cells, wherein the protein-immunoglobulin fusion may comprises a member of the TNF or TNF-receptor family, such as lymphotoxin-beta or lymphotoxin-beta receptor. Pharmaceutical compositions comprising the active protein-immunoglobulin fusions are also disclosed.