| 摘要 |
FIELD: biotechnology. ^ SUBSTANCE: method involves preparing plants-donors of meristems for freezing, wherein plants-donors are toughened on solid medium supplemented with substances of cytokinic effect; separating apexes and treating with cryogenic protectors; cooling apexes in cryogenic ampoules and providing cryogenic keeping; performing post-cryogenic plant regeneration from cryogenically kept meristems. Method for cryogenic keeping of meristematic apexes isolated from in vitro toughened plants allows plants to be recovered from cryogenically kept meristems of red raspberry (Rubus idaeus L.), with callus forming stage being avoided. ^ EFFECT: increased efficiency and simplified method. ^ 1 dwg, 2 tbl, 2 ex |
