RECOMBINANT DUFFY ANTIGENS, FUSIONS THEREOF, AND USE FOR DETECTION OF ANTIBODIES

摘要

Disclosed is a method of producing proteins that react with antibodies directed against Duffy blood group antigens Fya or Fyb. , This proteins comprise the extracellular domain of Fya or Fyb, fused to an expression protein, such as glutathione sulfotransferase. The protein or fusion antigen is immobilized onto an immobilization medium, and used to probe serum and plasma samples for the detection of anti-Fy(a) and anti-Fy(b) antibodies. A kit, containing the immobilized protein is disclosed. Further described are methods for the production of recombinant proteins and for the detection of antibodies in a sample.