发明名称 |
Identification of virulence determinants activators in prokaryotic pathogens |
摘要 |
Disclosed is a method for identifying activators of a transition metal-dependent repressor of virulence gene expression in infectious prokaryotic pathogens. The method utilizes genetic circuitry that represents the response of a given prokaryote to nutritional stress and the expression of genes that contribute to the establishment of the infectious process. The exposure of recombinant cells or a cell-free system containing the genetic circuitry to a non-metal ion test substance that activates the repressor produces a detectable response. The method is applicable for any prokaryote employing metal ion-dependent repressors to regulate specific gene expression, specifically as it pertains to virulence determinant expression.
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申请公布号 |
US2005058665(A1) |
申请公布日期 |
2005.03.17 |
申请号 |
US20040860727 |
申请日期 |
2004.06.03 |
申请人 |
BOSTON MEDICAL CENTER CORPORATION |
发明人 |
MURPHY JOHN R.;SUN LI |
分类号 |
C12N15/10;C12N15/63;C12Q1/68;(IPC1-7):C12Q1/68;A61K39/05 |
主分类号 |
C12N15/10 |
代理机构 |
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地址 |
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