GENETICALLY ALTERED ANTIBODY-PRODUCING CELL LINES WITH IMPROVED ANTIBODY CHARACTERISTICS
摘要
Dominant negative alleles of human mismatch repair genes can be used to generate hypermutable cells and organisms. Cells may be selected for expression of activation-induced cytidine deaminase (AID), stimulated to produce AID, or manipulated to express AID for further enhancement of hypermutability. These methods are useful for generating genetic diversity within immunoglobulin genes directed against an antigen of interest to produce altered antibodies with enhanced biochemical activity. Moreover, these methods are useful for generating antibody-producing cells with increased level of antibody production.
申请公布号
WO2005023865(A2)
申请公布日期
2005.03.17
申请号
WO2004US28905
申请日期
2004.09.03
申请人
MORPHOTEK, INC.;GRASSO, LUIGI;NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.
发明人
GRASSO, LUIGI;NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.