发明名称 |
THE SHORTER FRAGMENT OF 6-PHOSPHOFRUCTO-1-KINASE |
摘要 |
The invention deals with the shorter fragment of 6-phosphofructo-l-kinase (PFK1) with molecular mass between 49 and 55 kDa that is not significantly inhibited by citric acid and/or its salts and is post-translationally modified. The 49-52 kDa fragment that is formed by post-translational modifications of the fungal enzyme retains positive regulatory properties and is activated by specific activators, yet is no more sensitive to inhibition by citric acid a substance that in higher organisms acts as a feed back inhibitor. The invention describes a procedure that includes the introduction of the gene coding for a shorter fragment into the eukaryotic cells with an aim to increase the metabolic flow through the glycolysis and concomitantly to increase the rate of primary and secondary metabolites synthesis. The invention describes the use of the shorter fragment in biotechnological procedures for production of primary and secondary metabolites. |
申请公布号 |
WO2005023854(A2) |
申请公布日期 |
2005.03.17 |
申请号 |
WO2004SI00028 |
申请日期 |
2004.08.25 |
申请人 |
KEMIJSKI INSTITUT;LEGISA, MATIC;BENCINA, MOJCA |
发明人 |
LEGISA, MATIC;BENCINA, MOJCA |
分类号 |
C07K14/38;C12N9/12;C12N15/52 |
主分类号 |
C07K14/38 |
代理机构 |
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代理人 |
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地址 |
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