发明名称 |
Method for purifying calcium ion-binding protein |
摘要 |
The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid sample containing a calcium ion-binding protein and contaminants without any pretreatment such as addition of a chelating agent. More specifically, the present invention relates to a method for purifying a calcium ion-binding protein which comprises contacting said protein with a cation exchange carrier in the presence of calcium ions to let the said protein be adsorbed to the carrier, and after washing, eluting said protein, and to a calcium ion-binding protein having substantially no contaminants obtained by the method of the present invention.
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申请公布号 |
US2005059805(A1) |
申请公布日期 |
2005.03.17 |
申请号 |
US20040944810 |
申请日期 |
2004.09.21 |
申请人 |
JURIDICAL FOUNDATION THE CHEMO-SERO-THERAPEUTIC RESEARCH INSTITUTE;KOWA COMPANY, LTD. |
发明人 |
MIZOKAMI HIROSHI;FURUKAWA SHINICHI;SUGAWARA KEISHIN;ONCHI TATSUFUMI;KOMATSU KAZUHIRO;KOYANAGI SATOSHI;YOSHIZAKI HIDEO |
分类号 |
C07K14/47;C12N9/64;(IPC1-7):C07K14/47 |
主分类号 |
C07K14/47 |
代理机构 |
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