发明名称 |
RNA polymerase |
摘要 |
Disclosed are RNA polymerases consisting of a wild type RNA polymerase provided that at least one of amino acids in the wild type RNA polymerase has been modified to enhance its ability for incorporating 3'-deoxyribonucleotides and derivatives thereof in comparison with the corresponding wild type RNA polymerases. Specifically, disclosed are, for example, the RNA polymerases wherein at least one amino acid present in a nucleotide binding sites of the wild type RNA polymerases such as phenylalanine has been replaced with tyrosine. The RNA polymerases of the present invention are a RNA polymerase which exhibits little or no bias for incorporation between ribonucleotides and 3'-deoxyribonucleotide as well as among ribonucleotides having different base groups and among deoxyribonucleotides having. different base groups.
|
申请公布号 |
US6867027(B1) |
申请公布日期 |
2005.03.15 |
申请号 |
US19990254344 |
申请日期 |
1999.09.03 |
申请人 |
THE INSTITUTE OF PHYSICAL AND CHEMICAL RESEARCH;NIPPON GENE CO., LTD.;NIPPON GENETECH CO., LTD. |
发明人 |
HAYASHIZAKI YOSHIHIDE;WATAHIKI MASANORI |
分类号 |
C12N15/09;C12N1/21;C12N9/12;C12N15/54;C12Q1/68;C12R1/19;(IPC1-7):C12N9/12 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|