COMPOSITION FOR PROTECTING PROTEINS DEGRADATION COMPRISING SMALL HEAT SHOCK PROTEINS(SHSPS) AND METHOD OF TWO-DIMENSIONAL GEL ELECTROPHORESIS USING THE SHSPS

摘要

A composition for protecting proteins degradation comprising small heat shock proteins(sHSPs) and a method of two-dimensional gel electrophoresis using the same sHSPs are provided, thereby inhibiting reduction of protein spots when carrying out electrophoresis, so that a larger number of spots can be detected by the two-dimensional gel electrophoresis. The composition for protecting proteins degradation comprises the effective amount of small heat shock proteins(sHSPs), wherein the small heat shock proteins(sHSPs) has small molecular weight about 15 to 30 kDa. The method of two-dimensional gel electrophoresis using the sHSPs comprises the steps of: adding sHSPs into a protein mixture; and subjecting the protein mixture to two-dimensional gel electrophoresis, wherein the sHSP is one or more selected from IbpA, IbpB and IbpAB derived from E. coli, IbpA derived from Pseudomonas sp. and HSP26 derived from Saccharomyces cerevisiae; and the protein mixture is the total proteins of a certain cell of prokaryotes such as E. coli or Pseudomonas sp. or eukaryotes such as human.