| 摘要 |
FIELD: medicine. ^ SUBSTANCE: method involves culturing microorganisms in liquid nutrient medium, separating supernatant from microbial cells by centrifuging. Control is concurrently prepared as liquid nutrient medium. Then, each sample is treated with chloroform and separating supernatant from chloroform by centrifuging. Next to it, Staphylococcus aureus test culture is added to case and control samples, incubated, test culture cells are separated by centrifuging and microbial suspension is prepared from them. Hydrogen peroxide solution is added to each sample and they are incubated. The test culture cells are separated by centrifuging, nutrient medium is added and incubated. Optical density of grown-up cultures is measured in case and control samples and test culture biomass live weight gain in case sample is determined from formula P=[(ODCS - ODC ]/ ODC] *100%, where P is the biomass liveweight gain in case sample compared to control; ODC is the optical density of control test culture; ODCS is the optical density of case test culture. Then, conclusions are drawn if protective activity microorganisms are available in Fenton effect by interpreting test culture biomass live weight gain data in case sample. ^ EFFECT: wide range of functional applications in studying microbial cell physiology in medium containing small quantities of hydrogen peroxide. ^ 1 dwg, 2 tbl |
