| 摘要 |
Methods of obtaining a sample of target nucleic acid from cells containing the target nucleic acid and genomic DNA or RNA are disclosed. In contrast to prior art protocols, this method does not require the cells containing the target nucleic acid to be lysed and instead is based on the observation when cells are suspended in an aqueous medium and the target nucleic acid are released into the medium through the cell walls. The invention therefore helps to avoid the use of cell lysis, heating, extremes of pH, water immiscible solvents, and electrical fields used in prior art nucleic acid extraction methods. The present invention is particularly applicable to the separation of non-genomic nucleic acid, such as cellular vector DNA or RNA, self-replicating satellite nucleic acids or plasmid DNA, from genomic nucleic acids, such as host cell chromosomes and ribosomal RNA.
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