| 主权项 |
1.一种在分离的生物样品中体外检测蛋白存在的方法，该方法包括：a)将所述生物样品与对所述蛋白有特异性的抗体，在所述抗体和样品中的所述蛋白可以结合的条件下在进行体外接触，由此形成一种复合物，所述蛋白具有如下所述的氨基酸序列，并且由一段DNA片段编码，该DNA片段具有以下所示核苷酸序列中核苷酸79至1479的核酸序列，所述氨基酸序列为对应于所述核苷酸79至1479的氨基酸序列；<img file="C981167390003C1.GIF" wi="2740" he="234" />108 135 162TTG CCC TTC ACT TGT GGA AGA AAT GCA AAT GAA AAC AGC CCT AGG GAT GTT GAC GTT GGG GTG GCA CCT GCT GCA GAG GGTLeu Pro Phe Thr Cys Gly Arg Asn Ala Asn Glu Asn Ser Pro Arg Asp Val Asp Val Gly Val Ala Pro Ala Ala Glu Gly 189 216 243AAC GTG CAG CAT GTC GAG GGC AGC ACT GCC AAG GCT GGT TTG AGC TCA AGG TCA GGT GGA GGA GGT AGT CTC TCC CAT CTCAsn Val Gln His Val Glu Gly Ser Thr Ala Lys Ala Gly Leu Ser Ser Arg Ser Gly Gly Gly Gly Ser Leu Ser His Leu 270 297 324TTC TGC GAG TGC AGC TCT AAA CCC TGC CTG AAA CAC GTG GAG AAG CTA TCT GAG CTG CCT CCA GGA CAC ATG CAA ATG GACPhe Cys Glu Cys Ser Ser Lys Pro Cys Leu Lys His Val Glu Lys Leu Ser Glu Leu Pro Pro Gly His MET Gln MET Asp 351 378 405ACT CTG ATC ATA AAA TTA TCA GGA AGA TTG AGA AAT AAG ACA AAA ATG GAG GTG CCA CCA AAC CAG TGG AAA TTT TTC CCCThr Leu Ile Ile Lys Leu Ser Gly Arg Leu Arg Asn Lys Thr Lys MET Glu Val Pro Pro Asn Gln Trp Lys Phe Phe Pro 432 459 486TTT TCA TTC CTC TGG CAT TCC CTG GCC TTG ACT CAA GGC AGC CCA CAC TCT AGG AGC AGA CAC CAG GGC ACA GGT GGG GAGPhe Ser Phe Leu Trp His Ser Leu Ala Leu Thr Gln Gly Ser Pro His Ser Arg Ser Arg His Gln Gly Thr Gly Gly Glu 513 540 567CTC TGG GGG ACC CTC CAG GCT TAC TCA GTG AAT GGG TTA GCA GCA GCC ACA GGA GCC ACC ATG GAG CCT GCA GGG ACC CACLeu Trp Gly Thr Leu Gln Ala Tyr Ser Val Asn Gly Leu Ala Ala Ala Thr Gly Ala Thr MET Glu Pro Ala Gly Thr His 594 621 648AAC ACT GAG GGC AGG GAT CTT GCC TCT AAT CAG ATA AGC TGT GAT TCC CGA GAG GGT GGG GTA AAG GCC ACG GGT CTT TTTAsn Thr Glu Gly Arg Asp Leu Ala Ser Asn Gln Ile Ser Cys Asp Ser Arg Glu Gly Gly Val Lys Ala Thr Gly Leu Phe 675 702 729CTC TCC ACA TCT TCC CAC GTC ATG ACC CCA GAG GGT CGA AGA GGG AGA AAG TGT GAG CAC CGT GAC ATA ATG AGC CGC AGCLeu Ser Thr Ser Ser His Val MET Thr Pro Glu Gly Arg Arg Gly Arg Lys Cys Glu His Arg Asp Ile MET Ser Arg Ser 756 783 810CTT CTG ACT AGA TGC CCC AAA GAA GAA TCC CAG GTG ACC ACA CAG CAT CAG AGA AAC TGC AGG GTA ATG AGG AAC TTT GGALeu Leu Thr Arg Cys Pro Lys Glu Glu Ser Gln Val Thr Thr Gln His Gln Arg Asn Cys Arg Val MET Arg Asn Phe Gly 837 864 891AAG CAA TCC ATC GTG TTG TCA GTA AAA CCT CTG GCT CAC TCC CGA GCT GGG CAT GCA TGG ATG GTG ACC CTC GAT GGA ATALys Gln Ser Ile Val Leu Ser Val Lys Pro Leu Ala His Ser Arg Ala Gly His Ala Trp MET Val Thr Leu Asn Gly Ile 918 947 974GAC TAT GAG GAA CCA GGT GAG GGG ATC TAC CTC CAC CGA GAC GTG AGA GTG ACC TGC ATA CCC AAA CAC CAT GAG GCT TTAAsp Tyr Glu Glu Pro Gly Glu Gly Ile Tyr Leu His Arg Asp Val Arg Val Thr Cys Ile Pro Lys His His Glu Ala Leu 999 1028 1053AAG ACT GAG CTG ATG TGG AAG CCA CAG CCT CTG CAG GTT GCT CTG CAC TTG CAA CAT AAG CCC AAC CAC ATC AAT TGC TGCLys Thr Glu Leu MET Trp Lys Pro Gln Pro Leu Gln Val Ala Leu His Leu Gln His Lys Pro Asn His Ile Asn Cys Cys 1080 1107 1134AAA ACA AAA CTA CAG CAT TCT CCA TAC CAC TTA AAT AAG ACA CAG AGT CTC ACA ACA TTC AAA ACG CCC AGG ACA CAA TCCLys Thr Lys Leu Gln His Ser Pro Tyr His Leu Asn Lys Thr Gln Ser Leu Thr Thr Phe Lys Thr Pro Arg Thr Gln Ser 1161 1188 1215AAA ATT ACT TCT ACA AAA AAT CAG GAA AAT CTC AAT GAG CAA GGA AAA TGG CAA TCA GTA GCT GCC AGT GCT GAG ATG ACALys Ile Thr Ser Thr Lys Asn Gln Glu Asn Leu Asn Glu Gln Gly Lys Trp Gln Ser Val Ala Ala Ser Ala Glu MET Thr 1248 1270 1296ATG AGG GTT GGA ATC ATC AAC ATC TTT AAA GTA ATT ATC ATA AGC ATT CTC CAG CAA GTA ATG GCA AAC ACT CTT GAG ATAMET Arg Val Gly Ile Ile Asn Ile Phe Lys Val Ile ILe Ile Ser Ile Leu Glu Gln Val MET Ala Asn Thr Leu Glu Ile 1323 1350 1377AAT GGA AAG ATA AGA AGG CTC AGG GAG AAA GTG GAA TGT ACA AAG AAT GAC CAA GTG GGA ATT GCA CCA CTG GAA ACA AATAsn Gly Lys Ile Arg Arg Leu Arg Glu Lys Val Glu Cys Thr Lys Asn Asp Gln Val Gly Ile Ala Pro Leu Glu Thr Asn 1404 1431 1458CAC CAG GAT AAA GCA GTC TCT GGC TGG GCC AAC AGG AGA ATG GAA ATG AAA AGG GAA AGA GTT GTT ATG GCA GTT GTC CAAHis Gln Asp Lys Ala Val Ser Gly Trp Ala Asn Arg Arg MET Glu MET Lys Arg Glu Arg Val Val MET Ala VAl Val Gln<img file="C981167390005C1.GIF" wi="2757" he="443" />1647 1674 1701ATA CAA GAA TTA GCT GGG CAT GGT GGC AGG TGC CTG CAA TCC CAG CTA CTC AGG AGG CTG AGG CAG GAG AAT CAC TTG AACIle Gln Glu Leu Ala Gly His Gly Gly Arg Cys Leu Gln Ser Gln Leu Leu Arg Arg Leu Arg Gln Glu Asn His Leu Asn 1728 1755 1782TCG GGA GGT AGA GGG TGC AGT GAG CCA AAA TCG CAC CTC TGC ATT CCA GCC TGG GTG ACA GAG GGA GAC TCT GTC TCA AAASer Gly Gly Arg Gly Cys Ser Glu Pro Lys Ser His Leu Cys Ile Pro Ala Trp Val Thr Glu Gly Asp Ser Val Ser Lys 1809 1836 1863CAA AAC AAA ACA AAA AAT GAA CAG CAC CTC AGG AAC AAT ACC AAA AAG TCC AAC AGC TGT ATA ATT GGT GGC CCA GAA GGAGln Asn Lys Thr Lys Asn Glu Gln His Leu Arg Asn Asn Thr Lys Lys Ser Asn Ser Cys Ile Ile Gly Gly Pro Glu Gly 1890 1917 1944GAG GAG AAA GAG TGG AGT ACA GAA ATG AGA TCT GAA GAA CTA ATG ACT GAT AAT GTT TCA ATT TTG AAA AAG GAC ATA AACGlu Glu Lys Glu Trp Ser Thr Glu MET Arg Ser Glu Glu Leu MET Thr Asp Asn Val Ser Ile Leu Lys Lys Asp Ile Asn 1971 1998 2024CTA AAG ATT ATA GAT TCA AAA GCC CAG CTG AAT TCA AAT AGG ATA AAT ACA GAT GCA GAT ATA TTA TCA TTA AAC TGT GAALeu Lys Ile Ile Asp Ser Lys Ala Gln Leu Asn Ser Asn Arg Ile Asn Thr Asp Ala Asp Ile Leu Ser Leu Asn Cys Glu 2052 2079 2106ATA AAT TGG TTT TGT CAC AAG CCA GCA TTG TCA CTG TGG GAG AAA AGA GAT CAA AAG TAC ACA AGG AAG GAA GGA AAT ACAIle Asn Trp Phe Cys His Lys Pro Ala Leu Ser Leu Trp Glu Lys Arg Asp Gln Lys Tyr Thr Arg Lys Glu Gly Asn Thr<img file="C981167390006C1.GIF" wi="1948" he="57" />GAA TAT TAT GGC CAT GGG AAA GAG GTG TCA GTG TGA ATA CAT AGA ACA GCA CAC TTA AGC AAC AAC CCC AAA TGA TGG GGCGlu Tyr Tyr Gly His Gly Lys Glu Val Ser Val · Ile His Arg Thr Ala His Leu Ser Asn Asn Pro Lys · Trp Gly 2214 2241 2268TTC CTA CAA AAC AGT TGG CCT TTA CTC TTC AAA AGT GTC AGG TCA CGA AAT AAA TCC ATG CTG AGG ACC CGT TCC AGG TTAPhe Leu Gln Asn Ser Trp Pro Leu Leu Phe Lys Ser Val Arg Ser Arg Asn Lys Ser MET Leu Arg Thr Arg Ser Arg Leu 2295 2322 2349AAG CAG ACT AAA GGG GCT GGA CAA CAC AGT GAA ACG TGT GAG CTT GGA TTA GAT ATA TGC TGG ACT AGA GAA GGC TGT GAGLys Gln Thr Lys Gly Ala Gly Gln Pro Ser Glu Thr Cys Glu Leu Gly Leu Asp 2376 2457 2484GGG GAC AAT GGC TGA AAT GTG AAT GAG GTT ACT ATA GTT ATG AAA AAT GTT AAG ACT TGG AAA ATC TAT ATA AAG CAG ACG 2511 2538 2565GCA TAA TTC TTG TAC TTT TTT TGC AAC TTT TTA ATA AAC CTG AAA CTA TTT CAA AAT GAA AAG TTA ATC CAA GCT GTC TTG 2592 2619 2646AGT AGA AGT TAA AAC AAC AAC AAC AAA AGA AAA TTG AAA AGT TAA AAA TGA ACC CCC AAC AGA ATG TTC CCC TTT ATT TTT 2673 2700 2727CTT TCA TGT AAG GAC GCA GGA TAT GCA TTT TGC TCA GCT ACC ACC CTT CAC TGC ATC CCA TTT TGA GAA GTG GTA TTT TCT 2754 2781 2808TCA TTC ATC TGT TCT AGG TTT TTA AAA AAA TAT TTA AGA TCT TCT CTT TTT AAA GAA TCT GTT CAT TTG GAA TGT ACT TTT 2835 2862 2889TGC ATT TTT ACT TGT GAA AAT ATG TAT TTA TCC TTT TTG TTA TGA ATG TAT GAC TTC ACT GTG TCA GAG AAT ATG GTC TTA 2916 2943AGA GAT ACA GAA AAC TTT TGA GAA TGA TAA GAT CTG GAC ATG CTA GAT GAA ATC AAA GCC CTG GAT GTC CTT GTT CAA GCT Tb)检测所述复合物的存在。 |
