DIAGNOSTIC METHOD AND KIT OF CEREBROVASCULAR DISEASE BY ASSAYING RELATIONSHIP BETWEEN POLYMORPHISM OF GENE AND CEREBROVASCULAR DISEASE

摘要

PURPOSE: A diagnostic method and kit of cerebrovascular disease are provided, thereby easily identifying the genotype which has the high risk degree of cerebrovascular disease occurrence by assaying relationship between polymorphism of a certain gene and cerebrovascular disease, so that the cerebrovascular disease occurrence in a person who has the high risk degree of cerebrovascular disease occurrence can be prevented at an early stage. CONSTITUTION: The diagnostic method of cerebrovascular disease comprises the steps of: isolating DNA through a conventional method; verifying the GNB3 genotype of the isolated DNA by using PCR; culturing the PCR product under the appropriate condition; subjecting the cultured PCR product to agarose gel electrophoresis in tris-acetate-EDTA(TAE) buffer solution; staining the developed gel with ethidium bromide; and deciding the T/T genotype(homozygote) having a 268bp band as a genotype group having the high risk degree of cerebrovascular disease occurrence.