| 摘要 |
PURPOSE: A transformant for screening a HIV inhibitor harboring the chromosome integrated with foreign DNA is provided, thereby improving efficiency of HIV(human immunodeficiency virus) inhibitor screening method by using only one expression vector expressing a NC protein in comparison with the prior methods using two expression vectors. CONSTITUTION: The transformant for screening a HIV inhibitor harbors the chromosome containing a nucleotide sequence which can be transcribed into a psi RNA nucleotide sequence, and gene lacZ, sequentially, and an expression vector expressing NC protein. The method for screening the HIV inhibitor comprises the steps of: (i) culturing the transformant for screening the HIV inhibitor; (ii) treating the cultured transformant with a compound which is suspected as a HIV inhibitor; and (iii) measuring the expression level of beta-galactosidase from the compound treated transformant.
|
