| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for specifically detecting a target nucleic acid through developing an assaying system enabling a PCR (polymerase chain reaction) product to be specifically detected as it is in a double-stranded form. SOLUTION: This method for detecting the target nucleic acid in a specimen comprises the step(a) of amplifying the nucleic acid in the specimen using a pair of primers by a PCR reaction, wherein the pair of primers is so designed that a zinc finger protein recognition sequence present in the target nucleic acid is amplified by the PCR reaction, and the step(b) of detecting the PCR amplified product obtained in the step(a) using the zinc finger protein. As the zinc finger protein, Zif268 which recognizes, e.g. sequence 5'-GCGTGGGCG-3' can be used. COPYRIGHT: (C)2005,JPO&NCIPI
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