发明名称 |
СПОСОБЫ СЕКРЕТОРНОЙ ПРОДУКЦИИ БЕЛКОВ |
摘要 |
The object of the present invention is to provide a method of producing a heterologous protein by making a coryneform bacterium to produce and efficiently extracellularly secrete (secreto-production) an industrially useful heterologous protein. According to the present invention, a genetic construct is used where a gene sequence encoding an intended protein which is ligated to the downstream of a sequence encoding the signal peptide derived from a coryneform bacterium, the gene construct is introduced into a mutant coryneform bacterium which has a capacity of secreting the heterologous protein at least 2-fold higher than the wild type Corynebacterium glutamicum ATCC13869, the mutant coryneform bacterium is cultured and the extracellularly released heterologous protein is recovered. |
申请公布号 |
RU2003128977(A) |
申请公布日期 |
2005.02.27 |
申请号 |
RU20030128977 |
申请日期 |
2002.03.27 |
申请人 |
АДЗИНОМОТО КО., ИНК. (JP) |
发明人 |
КИКУТИ Йосими (JP);ДАТЕ Масайо (JP);УМЕЗАВА Юкико (JP);ЙОКОЯМА Кейити (JP);ХЕИМА Харуо (JP);МАЦУИ Хироси (JP) |
分类号 |
C12N15/12;C07K5/107;C07K14/195;C07K14/34;C07K14/36;C12N1/21;C12N15/09;C12N15/21;C12N15/77;C12P21/02;C12R1/15 |
主分类号 |
C12N15/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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