Biologisk funktionelt plasmid samt rekombinant plasmid indeholdende toluenmonooxygenase-gener, mikroorganismeværtsceller indeholdende eller transformeret med sådanne plasmider, DNA-segment kodende for toluenmonooxygenasegener samt fremgangsmåder.....

摘要

Disclosed and claimed are DNA gene segments, biologically functional plasmids and recombinant plasmids, and microorganism host cells containing such plasmids, all of which contain toluene monooxygenase genes from Pseudomonas mendocina KR-1 and which are useful in a method for the microbial bioconversion of selected phenyl compounds to selected phenolic compounds. In particular, the method is useful for making p-hydroxyphenylacetic acid which is a valuable chemical intermediate in the preparation of certain antibiotics and certain beta -adrenergic blocking agents.