РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК PCDNA-TCI, ОБЕСПЕЧИВАЮЩАЯ ЭКСПРЕССИЮ ИСКУССТВЕННОГО ГЕНА TCI В КЛЕТКАХ ЭУКАРИОТ, И РЕКОМБИНАНТНЫЙ АТТЕНУИРОВАННЫЙ ШТАММ БАКТЕРИЙ SALMONELLA ENTERITIDIS E-23/PCDNA-TCI КАК КАНДИДАТ ДЛЯ КОНСТРУИРОВАНИЯ ЖИВОЙ ДНК-ВАКЦИНЫ ПРОТИВ ВИРУСА ИММУНОДЕФИЦИТА ЧЕЛОВЕКА

摘要

FIELD: biotechnology, genetic engineering, molecular biology. ^ SUBSTANCE: invention proposes recombinant DNA pcDNA-TCI that has molecular mass 4.3 x 103 kDa and size 6 657 nucleotide pairs. The proposed recombinant plasmid DNA provides expression of artificial gene TCI comprising cytotoxic T-cellular epitopes of HIV-1 in eucaryotic cells. Also, invention proposes recombinant attenuated strain of bacterium Salmonella enteritidis E-23 pcDNA-TCI. The proposed strain is obtained by genetic transformation of the strain Salmonella enteritidis E-23 with plasmid pcDNA-TCI. The strain provides delivery DNA-vaccine in eucaryotic cells as the recombinant plasmid DNA pcDNA-TCI. Proposed groups of inventions provide inducing the expressed specific humoral and cellular immune response to HIV-1 in body. Proposed group of inventions can be used in medicine and virology for construction of live DNA-vaccine against HIV-1. ^ EFFECT: valuable biological and medicinal properties of strain and vaccine. ^ 2 cl, 3 dwg, 3 tbl, 5 ex