发明名称 |
GENETICALLY ENCODED BIOINDICATORS OF CALCIUM-IONS |
摘要 |
<p>The present invention relates to novel types of cellular calcium probes that are based on Troponin C and two chromophors suitable for FRET (fluorescence resonance energy transfer). The Troponin C-based calcium sensors of the invention function in diverse subcellular environments, for example even when tethered to a cellular membrane. The invention further provides nucleic acid constructs encoding the calcium probes of the invention, expression constructs, host cells and transgenic animals. Furthermore, methods for the detection of changes of local calcium concentrations and for detecting the binding of a small molecule to fragments of Troponin C are provided.</p> |
申请公布号 |
WO2005014636(A1) |
申请公布日期 |
2005.02.17 |
申请号 |
WO2004EP08739 |
申请日期 |
2004.08.04 |
申请人 |
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.;GRIESBECK, OLIVER;HEIM, NICOLA |
发明人 |
GRIESBECK, OLIVER;HEIM, NICOLA |
分类号 |
C07K14/435;C07K14/47;G01N33/542;G01N33/68;(IPC1-7):C07K14/47 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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