WEST NILE VIRUS VACCINE

摘要

It is intended to provide process for producing a West Nile Virus (WNV) vaccine and an attenuated WNV vaccine produced by this method. A process which comprises culturing an established cell line (Vero cells) originating in the kidney of a WNV-inoculated vervet monkey at a high density, attenuating the WNV-infected cells or a liquid culture medium thereof thus obtained, and then highly purifying WNV by sucrose density gradient centrifugation; WNV obtained by this process; and an attenuated WNV vaccine comprising the WNV or an antigenic component thereof as the main component and being substantially free from any nucleic acids or proteins originating in a host.