发明名称 |
Rapid identification of bacteria from positive blood cultures |
摘要 |
Disclosed is a method of detecting bacteria in a biological sample, especially a blood sample, without the need for extensive sub-culturing of the sample. Nucleic acid present within the sample is isolated and bacterial DNA specifically amplified using primers that uniquely prime the amplification of 16s rRNA-encoding nucleic acid. The amplicons are then digested with an endonuclease to yield a restriction fragment length profile for the biological sample. The restriction fragment length profile for the biological sample is then compare to a database of profiles made using cultures of known bacterial species. A match between the sample profile and the database quickly identifies the bacteria present in the sample.
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申请公布号 |
US2005037408(A1) |
申请公布日期 |
2005.02.17 |
申请号 |
US20040897592 |
申请日期 |
2004.07.23 |
申请人 |
CHRISTENSEN JEFFREY E.;SQUIRES PAMELA J.;REED KURT D. |
发明人 |
CHRISTENSEN JEFFREY E.;SQUIRES PAMELA J.;REED KURT D. |
分类号 |
C12P19/34;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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