METHODS AND COMPOSITIONS FOR TARGETED CLEAVAGE AND RECOMBINATION
摘要
Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.
申请公布号
WO2005014791(A2)
申请公布日期
2005.02.17
申请号
WO2004US25407
申请日期
2004.08.06
申请人
SANGAMO BIOSCIENCES, INC.;PABO, CARL, O.;URNOV, FYODOR;HOLMES, MICHAEL, C.;MILLER, JEFFREY, C.
发明人
PABO, CARL, O.;URNOV, FYODOR;HOLMES, MICHAEL, C.;MILLER, JEFFREY, C.