发明名称 |
METHODS OF PROTEIN PRODUCTION IN YEAST |
摘要 |
Vectors, host cells, and methods are provided for the production of proteins in yeast. The vectors generally contain a selection gene, a yeast 2 micron sequence, and a polynucleotide encoding a polypeptide, where the polynucleotide is operably linked to promoter, and where the polynucleotide contains one or more yeast-preferred codons. Host cells are cultured under conditions where, after an initial batch phase, oxygen concentration is kept high and glucose feed is regulated so that the yeast cells stay in respiratory metabolism. |
申请公布号 |
WO2005014825(A2) |
申请公布日期 |
2005.02.17 |
申请号 |
WO2004US25983 |
申请日期 |
2004.08.09 |
申请人 |
ARRIVA PHARMACEUTICALS, INC.;BARR, PHILIP, J.;GIBSON, HELEN, L. |
发明人 |
BARR, PHILIP, J.;GIBSON, HELEN, L. |
分类号 |
C07K14/81;C12N15/81 |
主分类号 |
C07K14/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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