| 摘要 |
The present invention relates to a method for analyzing gene expression which makes it possible, taking account of the translation state present in a cell type, tissue or organism, to correlate reliably the amount of mRNA transcribed from a gene to be investigated with the amount of protein translated from this mRNA. Determination of the translation efficiency of all mRNA variants which are transcribed from a gene to be investigated and which code for a particular protein makes it possible inter alia to identify the mRNA variant preferentially translated in a particular cell type, tissue or organism. On the basis of the amount and of the translation efficiency of the preferentially translated mRNA variant coding for a protein to be investigated it is possible to predict reliably the amount of the protein expressed in a cell type, tissue or organism.
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