Fremgangsmåde til bestemmelse af et kollagenbindende stof, især et adhæsionsproteins aktivitet

摘要

Method (A) for determining a collagen-binding substance in a sample, comprises: (a) covalently attaching reactive ("avid") collagen to a solid phase; (b) binding the collagen-binding substance to the collagen from the sample, and (c) determining the bound collagen-binding substance. Also claimed are: (1) a conjugate comprising reactive collagen covalently bound to a solid phase; (2) a device for performing the assay of (A), comprising the conjugate of (1); (3) a test kit for performing the assay of (A), comprising the device of (2) and a component comprising a standardised collagen-binding substance activity; (4) a process for producing the device of (2), comprising: (a) preparing a solution of reactive collagen; (b) chemically attaching the collagen to a solid phase, and optionally (c) freeze drying the product, and (5) a method for determining the physiological activity of vWF (von Willebrand factor) in a sample by binding it to immobilised collagen and detecting the bound vWF, characterised in that the vWF can be determined in the sample with a specific activity of≥40 (especially≥50) U/mg, protein.