METHOD OF SCREENING USEFUL PROTEIN

摘要

It is intended to provide a method of specifying a useful protein which comprises transferring cysteine residues at random into an amino acid sequence, thus synthesizing a protein having disulfide bonds at random and then analyzing the function of the protein. This object can be achieved by providing, for example, a screening method involving the following steps: (a) the step of preparing one or more mRNAs encoding a protein containing at least two cysteine residues and binding each of the obtained mRNA(s) to puromycin or a puromycin-like compound to give mRNA-puromycin conjugate(s); (b) the step of bringing the mRNA-puromycin conjugate(s) obtained in the step (a) into contact with a translation system to synthesize protein(s), thereby giving mRNA-puromycin-protein conjugate(s); and (c) the step of bringing the mRNA-puromycin-protein conjugate(s) obtained in the step (b) into contact with one or more target substances and judging whether or not any of the proteins in the mRNA-puromycin-protein conjugate(s) interacts with the target substance.