REGULATION OF RNA STABILITY

摘要

The present invention relates, e.g., to a method for screening an agent for the ability to modulate RNA processing. The agent is contacted with a sample comprising i) tristetraprolin (TTP) or a related protein, or an active fragment or variant thereof, ii) a poly(A) ribonuclease (PARN) polypeptide, or an active fragment or variant thereof, wherein, if the PARN is in a cell, the cell comprises exogenous PARN, and iii) an RNA which comprises an AU-rich element (ARE) downstream of a reporter sequence, and a 3' poly(A) tail, wherein processing of the RNA can be detected, under conditions effective for processing and, optionally, translation of the RNA; and a parameter associated with processing of the RNA is detected. The method can be conducted in a cell-free (in vitro) or cell-based format.