METHOD FOR LIGATING NUCLEIC ACIDS AND MOLECULAR CLONING
摘要
The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
申请公布号
EP1504119(A4)
申请公布日期
2005.02.09
申请号
EP20010918206
申请日期
2001.02.22
申请人
STRATAGENE CALIFORNIA
发明人
JI, HENRY;GREENER, ALAN, L.;SORGE, JOSEPH, A.;BAUER, JOHN;GIBBS, RICHARD;CARSTENS, CARSTEN-PETER