Method and kit for diagnosing the genetic predisposition of sheep to scrapie

摘要

The present invention relates to a method for determining the predisposition of sheep to scrapie by determining the presence or absence of the DNA sequences corresponding to one or more polymorphisms at sites 136, 154 and 171 of the prion protein, comprising the steps of isolating the DNA from uncoagulated blood and amplifying one or more primers in such a way as to generate amplified products complementary to the said polymorphic sites, and is characterized in that the primers generating the amplified products are labelled with one or more labels, and the said labelled amplified products are subsequently hybridized on strips to oligonucleotide probes corresponding to the said polymorphic sites. A kit for applying the method according to the invention is also described.