发明名称 |
Measurement of fluorescence using capillary isoelectric focusing |
摘要 |
Improved apparatus is disclosed for carrying out axially illuminated laser induced fluorescence whole-column imaging detection in the capillary isoelectric focusing of proteins. The separation capillary was made of low refractive index Teflon conditioned with methylcellulose to reduce electroosmotic flow and a small amount of high refractive index organic solvent (glycerol) was added to the sample mixture. It was found that an axially directed laser excitation beam was propagated essentially with total internal reflection, so that minimum interference arose from stray light or from scattering light originating from the wall of the capillary. With the naturally fluorescent protein R-phycoerythrin, a concentration detection limit LOD 10<-11 >M or mass LOD 10<-17 >Mo was obtained.
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申请公布号 |
US6852206(B2) |
申请公布日期 |
2005.02.08 |
申请号 |
US20010832807 |
申请日期 |
2001.04.12 |
申请人 |
PAWLISZYN JANUSZ |
发明人 |
PAWLISZYN JANUSZ;WU XING-ZHENG;HUANG TIEMIN |
分类号 |
G01N21/64;G01N27/447;(IPC1-7):C02F1/469 |
主分类号 |
G01N21/64 |
代理机构 |
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代理人 |
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地址 |
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