PREPARATION OF IMMUNOLOGICALLY ACTIVE SUBSTANCE CAPABLE OF ACTIVATING IMMUNE-RELATED CELLS AND IMPROVING ACTIVITY OF LYMPHOCYTE DIVISION-PROMOTING MATERIAL BY CULTURING BASIDIOMYCETES IN CULTURE MEDIUM CONTAINING RICE BRAN

摘要

PURPOSE: A method of preparing an immunologically active substance by culturing Basidiomycetes in a culture medium containing rice bran and then fractioning polysaccharides exhibiting immunoactivity from the culture solution is provided. The substance can activate immune-related cells such as immune cells and macrophages and improve activity of lymphocyte division-promoting material. CONSTITUTION: The immunologically active substance is prepared by culturing Basidiomycetes in a culture medium containing 1 to 20%(w/v) of rice bran at 15 to 35deg.C for 1 to 3 days while agitating at 120rpm and fractioning polysaccharides exhibiting immunoactivity from the culture solution. Basidiomycetes are Manascus pilosus or Aspergillus oryzae. The culture medium contains 2%(w/w) of rice bran, 5%(w/w) of peptone and 0.1%(w/w) of KH2PO2 and has a pH of 5.0.