发明名称 |
METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES |
摘要 |
<p>This invention provides a method for combining overlapping DNA molecules comprising: (a) providing first and second DNA fragments, the first having a region homologous to a region in the second; (b) tagging the first DNA fragment with a selectable marker; (c) cloning the first DNA sequence into a retrieval vector to form a DNA-vector complex; (d) linearizing the DNA-vector complex; and (e) inserting the first DNA fragment from the DNA-vector complex into the second DNA fragment using homologous recombination to form a combined DNA molecule; and (f) removing the selectable marker, thereby generating a combined DNA molecule. The invention further provides a vector for retrieving and inserting a selected DNA molecule into a target DNA molecule.</p> |
申请公布号 |
WO2005010179(A1) |
申请公布日期 |
2005.02.03 |
申请号 |
WO2004CN00858 |
申请日期 |
2004.07.23 |
申请人 |
THE UNIVERSITY OF HONG KONG |
发明人 |
HUANG, JIANDONG;TANNER, JULIAN, ALEXANDER;ZHANG, XINMEI |
分类号 |
C12N15/10;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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