CARBON NANOTUBE FILM HAVING HIGH DENSITY AND METHOD FOR PRODUCING BIOCHIP USING THE SAME TO REDUCE UNNECESSARY SIGNALS IN GENOTYPING, MUTATION DETECTION AND PATHOGEN IDENTIFICATION

摘要

PURPOSE: A carbon nanotube(CNT) film having high density and a method for producing a biochip using the same are provided to reduce unnecessary signals as compared to the conventional biochips, in genotyping, mutation detection, pathogen identification and the like. CONSTITUTION: The carbon nanotube film having high density, which has exposed carboxylic groups on the surface, is produced by: (a) reacting a CNT having exposed carboxylic groups with a substrate where amine groups are exposed, to form a CNT monolayer on the surface of the substrate through amide bonds between amine groups and carboxylic groups; (b) reacting the CNT monolayer with diamine organic compounds to form a layer of organic amines on the CNT monolayer, and then reacting the CNT having exposed carboxylic groups with the organic amine to layer the CNT; and (c) repeating the above step (b) n times, so as to form high density CNT film having exposed carboxylic groups with alternately layered CNT and organic amine as much as the number of n. The method for producing a biochip using the CNT film comprises the steps of fixing a bioreceptor having a functional group, which can bond with the exposed carboxylic groups chemically or physicochemically, wherein the bioreceptor is enzyme substrates, ligands, amino acids, peptides, proteins, nucleic acids, lipids or carbohydrates.