发明名称 NON-STOCHASTIC GENERATION OF GENETIC VACCINES AND ENZYMES
摘要 This invention provides methods of obtaining novel polynucleotides and encod ed polypeptides by use of non-stochastic methods of directed evolution (DirectEvolution.TM.). These methods include non- stochastic polynucleotide site-saturation mutagenesis (Gene Site Saturation Mutagenesis.TM.) and non-stochastic polynucleotide reassembly (GeneReassembly.TM.). Through use of the claimed methods, genetic vaccines, enzymes, and other desirable molecules can be evolved towards desirable properties. For example, vaccine vectors can be obtained that exhibit increased efficacy for use as genetic vaccines. Vectors obtained by using the methods can have, for example, enhanced antigen expression increased uptake into a cell, increased stabilit y in a cell, ability to tailor an immune response, and the like. This invention provides methods of obtaining novel enzymes that have optimiz ed physical and/or biological properties. Furthermore, this invention provides methods of obtaining a variety of novel biologically acti ve molecules, in the fields of antibiotics, pharmacotherapeutics, and transgenic traits.
申请公布号 CA2325351(C) 申请公布日期 2005.02.01
申请号 CA20002325351 申请日期 2000.02.04
申请人 DIVERSA CORPORATION 发明人 SHORT, JAY M.
分类号 C12N15/09;A61K38/00;A61K39/00;A61K39/39;A61K48/00;A61P33/06;C07K14/445;C12N1/15;C12N1/19;C12N1/21;C12N5/02;C12N5/10;C12N9/00;C12N9/16;C12N9/24;C12N9/38;C12N15/01;C12N15/10;C12P21/02;C12Q1/02;C12Q1/68;G01N33/15;G01N33/50;G01N33/53;G01N33/566;(IPC1-7):C12N15/10;C12P21/00;C12P19/34;A61K31/70 主分类号 C12N15/09
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