METHOD FOR MANUFACTURING BIOSENSOR HAVING IMPROVED STABILITY USING HIGH DENSITY CARBON NANOTUBE PATTERN

摘要

PURPOSE: A method for manufacturing a biosensor using a high density carbon nanotube(CNT) pattern is provided, thereby improving stability of the biosensor by environment changes. CONSTITUTION: The method for manufacturing a high density carbon nanotube pattern comprises the steps of: (a) reacting a substrate in which amino groups are exposed on the surface with CNT in which carboxyl groups are exposed on the surface to form amine bonds between the amino groups and carboxyl groups, thereby forming a CNT monolayer on the surface of the substrate; (b) reacting the CNT monolayer with diamine organic compounds to form an organic amine layer on the CNT monolayer, and reacting the organic amine with carboxyl group exposed CNT to accumulate CNT; and (c) repeating the step (b) n times to reciprocally accumulate the CNT layer and organic amine layer n times, thereby forming the carboxyl group exposed high density CNT pattern. The CNT biosensor is prepared by chemically or physicochemically binding a bioreceptor with a chemical function group to the carboxyl group exposed high density CNT pattern which is fixed on the substrate with high density, wherein the bioreceptor is enzyme substrate, ligand, amino acid, peptide, protein, nucleic acid, lipid, cofactor or carbohydrate.