Recombinant lipidated PsaA protein, methods of preparation and use

摘要

A hybrid nucleic acid molecule comprising a first nucleic acid sequence encoding a signal sequence of a lipoprotein other than PsaA and a second nucleic acid sequence encoding a mature PsaA protein, or fragment thereof, wherein the signal sequence of the lipoprotein, preferably OspA of a Borrelia species, is contiguous with the second nucleic acid sequence.; a process for the production of a recombinant lapidated PsaA protein; a recombinant PsaA protein produced by the process; an immunological composition comprising the recombinant psaA protein; the use of the immunological composition for the manufacture of a medicament for immunizing a host against pneumococcal infection; and an immunogenic composition for intranasal administration to a host susceptible to pneumococcal carriage to elicit a protective immunological response against colonization with Streptococcus pneumoniae in the nasopharynx, which comprises an immunizing amount of recombinant lapidated PsaA. (62) Divided out of 506428