СПОСОБ ПОЛУЧЕНИЯ ДИАГНОСТИКУМА ДЛЯ ОПРЕДЕЛЕНИЯ ЭНДОТОКСИНА БАКТЕРИЙ РОДА SERRATIA

摘要

FIELD: medicine, veterinary and food processing industry. ^ SUBSTANCE: claimed method includes manufacturing of corn-shell-type latex, wherein corn represents polystyrene latex and shell represents styrene-zinc methacrylate copolymer latex in repeating unit mass ratio of 1:0.8-1:0.4. Then latex is sensitized with monoclonal antibodies of lgG2a and lgG2b fractions in 0.01 M glycine buffer at pH 7.2-8.8 without preliminary conditioning followed by incubation primarily at 37-39°C for 90-159 min and further at 10°C for 10 hours. Obtained diagnosticum has specificity of 99±0.3, sensibility up to 10 pg/ml and makes it possible to obtain investigation results for 10 min or less. ^ EFFECT: accelerated method for diagnosticum production. ^ 4 tbl