发明名称 |
Intron fusion construct and method of using for selecting high-expressing production cell lines |
摘要 |
This invention relates to a DNA construct, methods of selecting for high-expressing host cells, a method of producing a protein of interest in high yields and a method of producing eukaryotic cells having multiple copies of a sequence encoding a protein of interest. In one method, stable clones capable of producing a high level of a product of interest are generated from one step of a direct selection immediately after transfection.
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申请公布号 |
US2005019925(A1) |
申请公布日期 |
2005.01.27 |
申请号 |
US20030715270 |
申请日期 |
2003.11.14 |
申请人 |
GENENTECH, INC. |
发明人 |
KRUMMEN LYNNE ANN;SHEN AMY YIJUAN;CHISHOLM VANESSA |
分类号 |
C12N1/15;C12N1/19;C12N15/85;C12N15/90;(IPC1-7):C12N15/63;C12N15/87 |
主分类号 |
C12N1/15 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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