发明名称 METHOD FOR PRODUCING ANTI-2,4-DICHLOROPHENOL MONOCLONAL ANTIBODY AND METHOD FOR QUANTITATIVELY DETERMINING ANTIGEN BY USING THE ANTIBODY
摘要 PROBLEM TO BE SOLVED: To provide an antibody against 2,4-dichlorophenoxyacetic acid and its related compound for environmental monitoring. SOLUTION: A large amount of 2,4-dichlorophenoxyacetic acid (2,4-D) as a herbicide has long been applied to soil and is therefore accumulated in a large amount in soil. 2,4-D is slowly metabolized into 2,4-dichlorophenol (2,4-DP) and is then accumulated. Although 2,4-DP is an important marker compound for grasping the historical background of pollution of soil or water with 2,4-D, a method for analyzing the compound in high sensitivity is not yet developed. A technique of simply and highly reproducibly assaying 2,4-DP amd 2,4-D in a concentration of several ng/ml is developed by making an anti-2,4-DP monoclonal antibody and establishing a competitive ELISA (enzyme-linked immunosorbent assey). COPYRIGHT: (C)2005,JPO&NCIPI
申请公布号 JP2005023015(A) 申请公布日期 2005.01.27
申请号 JP20030189538 申请日期 2003.07.01
申请人 MASAYAMA YUKIHIRO 发明人 TANAKA HIROYUKI;MASAYAMA YUKIHIRO;MIURA NORIO
分类号 G01N33/53;C07K16/44;C12P21/08;G01N33/00;(IPC1-7):C07K16/44 主分类号 G01N33/53
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