发明名称 |
Ligand binding assays for vanilloid receptors |
摘要 |
The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.
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申请公布号 |
US2005019958(A1) |
申请公布日期 |
2005.01.27 |
申请号 |
US20040922683 |
申请日期 |
2004.08.19 |
申请人 |
ZHANG SUI-PO;CODD ELLEN E. |
发明人 |
ZHANG SUI-PO;CODD ELLEN E. |
分类号 |
G01N33/543;G01N33/53;G01N33/566;(IPC1-7):G01N33/536 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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