PROTEIN DESIGN FOR RECEPTOR-LIGAND RECOGNITION AND BINDING

摘要

We describe processes for the protein structure-based design or redesign of receptor-ligand interfaces (ligand-binding sites) in which a ligand is recognized and bound. Receptors designed in this manner can then be synthesized artificially or naturally, or used to engineer cells, tissues, or organisms. They can be further evaluated by empirical methods (e.g., ligand recognition and binding, signaling, catalysis), subjected to further improvement, and/or the process can be iterated in multiple cycles (e.g., consideration of quantitative structure-activity relationship data).