发明名称 |
Sequential generation of multiple chemiluminescent signals on solid supports |
摘要 |
Chemiluminescent assays to determine the presence and/or amount of one or more labeled analytes in a sample are described wherein a solid support is contacted sequentially with first and second chemiluminescent substrates each of which are capable of being activated by an enzyme and the resulting chemiluminescent signals are detected. A plurality of probes are disposed on a surface layer of the solid support in a plurality of discrete areas. Some of the probes are bound to a conjugate of the first enzyme and some of the probes are bound to a conjugate of the second enzyme. The assay can be used to compare biological samples (e.g., mRNA populations from different cells) on the same support surface. Alternatively, one of the chemiluminescent signals generated can be used as a control signal for normalizing the chemiluminescent assay data.
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申请公布号 |
US2005019778(A1) |
申请公布日期 |
2005.01.27 |
申请号 |
US20030620332 |
申请日期 |
2003.07.17 |
申请人 |
VOYTA JOHN C.;SMITH ROBERT M.;SCHROTH GARY P.;SPARKS ALISON L.;EDWARDS BROOKS N.;GONZALEZ CAROLYN |
发明人 |
VOYTA JOHN C.;SMITH ROBERT M.;SCHROTH GARY P.;SPARKS ALISON L.;EDWARDS BROOKS N.;GONZALEZ CAROLYN |
分类号 |
C12Q;C12Q1/68;G01N33/53;G01N33/535;G01N33/537;G01N33/543;G01N33/545;G01N33/552;G01N33/58;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q |
代理机构 |
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地址 |
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