РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК P30NE2, КОДИРУЮЩАЯ 181-АМИНОКИСЛОТНЫЙ N-КОНЦЕВОЙ ФРАГМЕНТ ГЛИКОПРОТЕИНА E2 ВИРУСА КЛАССИЧЕСКОЙ ЧУМЫ СВИНЕЙ И ОБЕСПЕЧИВАЮЩАЯ ЕГО ЭКСПРЕССИЮ В КЛЕТКАХ БАКТЕРИЙ E.COLI

摘要

FIELD: genetic engineering. ^ SUBSTANCE: recombinant plasmid DNA p30NE2 contains DNA fragment of pasmid pQE30, including promoter/operator of T5 phage; ribosome-binding site; phage lambda transcription terminator, and beta-lactomase gene as genetic marker, as well as BamHI/HindIII fragment, encoding 181-amino acid N-terminal fragment of glycoprotein E2 of classical bog cholera virus. Plasmid provides expression of glycoprotein E2 of classical bog cholera virus in E/coli bacteria cells. Invention is useful in diagnostic investigation and in vaccine production. ^ EFFECT: genetically engineered plasmid for vaccine preparations. ^ 3 dwg, 6 ex