发明名称 |
DNA isolation method |
摘要 |
Disclosed is a method and apparatus for the isolation of DNA or cell nuclei or a mixture thereof from cell samples in a CD device. The method includes treating a suspension of whole cells with a lysis reagent so as to lyse the cytoplasmic membrane and at least some of the nuclear membranes, and introducing the lysate into micro-channels of a microfabricated apparatus in which each of the micro-channels is provided with a barrier disposed in the channel to impede the passage or flow of DNA and cell nuclei while allowing the passage of liquid through the micro-channel so that a mesh comprising DNA is formed in the channel.
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申请公布号 |
US2005019819(A1) |
申请公布日期 |
2005.01.27 |
申请号 |
US20040924151 |
申请日期 |
2004.08.23 |
申请人 |
TOOKE NIGEL ERIC;THOMAS PHILIP LANDEG;KENRICK MICHAEL KENNETH |
发明人 |
TOOKE NIGEL ERIC;THOMAS PHILIP LANDEG;KENRICK MICHAEL KENNETH |
分类号 |
C12N15/09;C12M1/00;C12N5/06;C12N15/10;(IPC1-7):C12Q1/68;C07H21/04;C12M1/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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