| 摘要 |
The present invention provides nucleic acid hybridization probes having a target-binding region and a labeled hairpin structure at at least one end of the probe. The hairpin-labeled probes include oligonucleotides, dendrimers, and primer-extended nucleic acids. The probes can be used in disclosed metho ds for detection of target nucleic acids. In addition, the oligonucleotide prob es can be used in disclosed methods for primer-extension, including, e.g., rand om priming and PCR amplification, to produce the primer-extended hairpin-labele d probes. Also disclosed are kits comprising the haipin-labeled oligonucleotid e and dendrimer probes. Further, the present invention provides biomolecules (e.g., peptides, polypeptides, carbohydrates, lipids, and the like) that are labeled via linkage to labeled hairpin structures.
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