| 摘要 |
PURPOSE: An ELISA screening system for potential drugs that can improve obesity and type 2 diabetes is provided, thereby simply and rapidly screening the potential drugs that can improve obesity, type 2 diabetes. CONSTITUTION: A transformant Escherichia coli DH5@/PGEX4T-1-PPARγ2 (KCTC 10190BP) producing GST-PPARγ2(glutathione s-transferase-peroxisome proliferated activated receptor gamma isoform 2) is produced by transforming E. coli DH5@ with pGEX4T-1 containing human PPARγ2(peroxisome proliferated activated receptor gamma isoform 2) gene. A transformant E. coli BL21(DE3)/pET28a-SRC-1(633-708a.a) (KCTC 10191BP) producing SRC-1(steroid receptor co-activator01) is produced by transforming E. coli with pET28a containing SRC-1 gene. A screening method for potential drugs that can improve obesity and type 2 diabetes comprises analyzing the binding activity between the GAS-PPARγ2 protein and SRC-1 soluble fraction using ELISA.
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