Culture medium containing chicken serum and inactivated fibroblasts, used to grow keratinocytes for production of attenuated virions, for preparation of vaccines

摘要

Culture medium (A) comprises chicken serum and a culture substrate of inactivated fibroblasts. Independent claims are also included for the following: (1) keratinocyte culture that comprises (A); (2) method for culturing epidermal cells and keratinocytes that comprises inoculating (A) with a suspension of keratinocytes then incubation; (3) process for propagating a virus in vitro by culturing virus-infected keratinocytes by method (2) and extracting the virions formed; (4) enveloped, live but attenuated DNA virions produced by method (3); and (5) live, attenuated vaccines containing at least one virion of (4) and a vaccine carrier. ACTIVITY : Virucide; Cytostatic. No details of tests for these activities are given. MECHANISM OF ACTION : Vaccine.