RNA-CLEAVING DNA ENZYMES WITH ALTERED REGIO- OR ENANTIOSELECTIVITY

摘要

The present invention provides catalytic single-stranded DNA molecules, and methods using the same, that have site-specific endonuclease activity that is specific for a cleavage site in a substrate nucleic acid sequence, that includes a non-naturally occurring single-stranded ribonucleic acid. The catalytic DNA molecule includes one or more loop regions and one or more binding regions, wherein the binding regions bind to complementary sequences of the substrate nucleic acid sequence. These non-naturally occurring single-stranded nucleic acids include a 2',5' linked residue or an L-enantiomer residue.