发明名称 |
A METHOD OF SELECTIVELY PRODUCING MALE OR FEMALE STERILE PLANTS |
摘要 |
<p>A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/ or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male, or female reproductive structures and the non-phytotoxic substance is a D-alpha amino acid, or a peptide derivative of a non-protein D-alpha amino acid, characterised in that the enzyme is a mutant D-amino acid oxidase, obtainable from Rhodotorula gracilis, which oxidase comprises a lysine at position 58 rather than a phenylalanine in the wild type sequence.</p> |
申请公布号 |
WO2005005641(A1) |
申请公布日期 |
2005.01.20 |
申请号 |
WO2004GB02447 |
申请日期 |
2004.06.09 |
申请人 |
SYNGENTA LIMITED;DALE, RICHARD;HAWKES, TIMOTHY, ROBERT;THOMPSON, PAUL, ANTHONY;VINER, RUSSELL |
发明人 |
DALE, RICHARD;HAWKES, TIMOTHY, ROBERT;THOMPSON, PAUL, ANTHONY;VINER, RUSSELL |
分类号 |
C12N9/06;C12N15/82;(IPC1-7):C12N15/82 |
主分类号 |
C12N9/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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